Item #: CS DGGE
Our Price: $4,200.00
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Description  more details

VS20WAVE-DGGE Features

  • Maximum 96-sample throughput compatible with microplates and thermal cycler blocks.
  • Four-screw vertical clamping technology accelerates set up
  • Large format 20x20cm glass plates for improved resolution
  • 100ml gradient mixer, with valve-controlled 50ml reservoir and mixing chambers, makes two 1mm parallel denaturing gradient gels
  • Microprocessor-controlled temperature control unit accurate to 0.02C

Denaturing Gradient Gel Electrophoresis (DGGE) is an important technique used in the search for mutations and DNA polymorphisms critical in genetic disorders and cancers, and to understand genetic diversity among species. The new omniPAGE VS20WAVE-DGGE is a flexible system that can be fully customised to perform several different mutation detection techniques. The VS20WAVE-DGGE is a versatile single-unit solution for different single-base pair mutation detection methods. The newly designed VS20WAVE-DGGE is a complete system for DNA mutation analysis.

Using the innovative vertical screw-clamp technology of the new VS20-WAVE system, the VS20WAVE-DGGE is fully equipped with temperature control unit, stirrer, and gradient mixer and casting accessories to perform specific mutation analysis applications. A powerful microprocessor-controlled PID temperature control unit enables various mutation detection techniques to be undertaken between ambient temperature and 70C, while the simple fourscrew design of the WAVE insert accelerates set up of denaturing PAGE gels. Accordingly, the new VS20-DGGE can be used to screen single-base pair changes in the following applications:

Heteroduplex analysis (HA)

Parallel Denaturing Gradient Gel Electrophoresis (DGGE)

Constant Denaturing Gradient Gel Electrophoresis (CDGE)

 

The flexibility provided by the modular design of the VS20WAVE-DGGE and its wide range of accessories enables laboratories to switch quickly and easily between different mutation detection techniques, thereby maximising throughput and screening efficiency. A maximum 96-sample throughput allows detection of as many mutations within a couple hours, alleviating many of the bottlenecks associated with screening for DNA mutations. -

Benefits Include
  • Maximum 96 Sample Throughput-
    using two 48-sample combs- allows samples to be transferred quickly and easily to each gel from standard 96-well microplates or thermal cycler blocks
  • Large Format-
    20 x 20cm glass plates allow gradient gels to be poured containing wider ranges of denaturant concentration, maximizing sensitivity and resolution
  • 100ml gradient mixer-
    used with the VS20 casting base to make two 2mm parallel denaturing gradient gels
  • can also be adapted for constant denaturing gel electrophoresis (CDGE)-
    if the denaturent concentration required for partial melting of the DNA target sequence is already known

Parallel DGGE Overview:
Parallel DGGE is based on the application of a denaturant concentration gradient - increasing in the direction of electrophoresis within a polyacrylamide gel - and uniform temperature to induce the partial unwinding or melting of double-stranded DNA by distinct domains in a sequence-specific manner. Melting breaks the hydrogen bonds holding together each base pair within a DNA sequence: with domains rich in GC base pairs melting at higher temperatures than those with high A-T base-pair content.

Incorporating sequence high in G-C content into the DNA target sequence by PCR® amplification (GC-clamping) prevents the DNA target with the lowest melting domain from melting completely at its predetermined Tm, which is maintained by the application of a constant temperature across the gel and within the buffer. Because both gel temperature and increasing denaturant concentration act to melt DNA in a sequenceand domain-specific manner, any point mutations amplified within a PCR® product will affect its Tm so that its subsequent mobility within a polyacrylamide gel differs from its wild type counterpart (see schematic diagram).

Consequently, the sensitivity of this technique in distinguishing individual DNA sequences, which might differ only by a single point mutation, has proven highly attractive to scientists screening for unknown mutations.

Complete System Includes:

  • Twin-plate maxi-gel unit with GRM, heated gel tank with PT100 temperature sensor and thermometer, lid,>
  • 2 x (20 x 20cm; W x H) plain glass plates
  • 2 x (20 x 20cm) notched glass plates, 4 x 1mm spacers,
  • 2 x spacer aligners and 2 x 1mm thick 24-sample combs,
  • 2 x silicone seals and 1 x dummy plate;
  • plus GM100 gradient mixer and heat sensor control unit
Technical Specifications DGGE Denaturing Gradient Gel Electrophoresis
Max number of Gels 2 per run
Gel Dimensions WxH 16 x 17.5 cm
Plate Dimensions 20 x 20 cm
Unit Dimensions 40.4 x 17 x 44 cm
Weight 8 kg
Max Sample Capacity 96 samples, 48 per gel
Buffer Volumes 8.5 L
Combs Available

1, 1.5, 18MC, 24, 36, & 48 samples
0.75, 1.0, 1.5 & 2mm thickness

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