The most versatile, self-contained system on the market for real-time horizontal gel electrophoresis
• System includes everything except chemicals and reagents to run horizontal SYBR- and EtBr-stained gels in real-time
• Combines the flexibility and high resolution capability of the MSCHOICE system with the time- and space-saving convenience of having the power supply and gel illuminator integrated within one highly compact bench top unit
• Optional Gel Documentation System with 10 megapixel digital camera fits directly over the base unit and gel tank to allow gels to be imaged either as bands migrate in real-time or at the end of the electrophoresis run
• Includes accessories for standard gel electrophoresis and for size fractionation and simplified real-time gel free DNA band extraction and purification
Real-time size fractionation and recovery of nucleic acids
runVIEW is an innovative new system which can be used to maximise the efficiency of DNA recovery from EtBr and SYBR stained gels by minimising the number of steps involved in postelectrophoretic purification. runVIEW consists of a multiSUB MSCHOICE system with bluVIEW lid, containing an orange spectral emission filter within its viewing pane, and a base unit with integrated power supply and blue LED gel illuminator.
Simple DNA recovery
Using runVIEW is simple: first, cast a gel featuring two rows of wells with one matching pair of the preparatory combs supplied, before transferring the blue-light transparent gel tray to the MSCHOICE tank located on the base unit. Add sufficient buffer just to cover the gel, and remove the combs to load the DNA samples into the upper row of wells (‘Loading’ tier). Replace the lid to connect the MSCHOICE tank to the integrated power supply before applying the voltage and switching on the blue LED gel illuminator. Watch through the bluVIEW lid’s viewing pane as the samples migrate in real-time to the second row of wells (‘extraction’ tier). Once the DNA bands of interest enter the ‘extraction’ tier, simply stop the power supply, remove the lid and harvest the DNA by pipette.
Track DNA without harmful UV
Blue light is safe and has none of the detrimental effects of UV transillumination, including mutagenesis which can compromise cloning effi ciency, while runVIEW’s capacity to provide real-time visualisation of electrophoresis enables DNA to be tracked as it migrates through the gel. This allows the user to judge precisely when the band of interest is ready for extraction.
Save on time-consuming gel elution techniques
Running DNA into a well containing buffer effectively purifi es the DNA of agarose, eliminating the need for time-consuming gel excision and purifi cation techniques that also result in sample loss. Once harvested, the DNA needs only to be cleaned by a straightforward ethanol precipitation when it is ready for further digestion or ligation.
No expensive commercial gels
runVIEW works with standard EtBr, SYBR Green and SYBR Safe gels cast within the 15x7, 15x10 or 15x15cm MSCHOICE gel trays, and therefore does not require expensive precast gels and accessories.
A self-contained system
The base unit, which houses the in-built power supply and blue LED gel illuminator, is compact, dual-voltage and portable, and allows electrophoresis, gel visualisation and extraction to be performed at the bench, without the inconvenience of having to transport gels to a darkroom elsewhere within the laboratory.
Other benefi ts offered by runVIEW include:
• Power supply integrated within the base unit – adjustable in precise 1V or 1mA increments to a maximum 150V or 300mA constant voltage or current output; timer function to 999 minutes for extended runs.
• Specialist combs for specialist applications - double-sided 1mm preparatory combs (1- / 2-sample and 4- / 16-sample standard) included for nucleic acid recovery, plus four multichannel compatible 20- / 28-sample combs for rapid screening of nucleic acids from 96-well thermal cycler blocks and microtitre plates.
• Get real-time - existing MSCHOICE users may customise their system by purchasing the base station and bluVIEW lid.
• Versatile - Both power supply and gel illuminator can be used with other gels and electrophoresis systems.
CONFIGURATIONS
CSL-RVMSCHOICE7
runVIEW system complete with 15 x 7cm gel tray, 1 set of casting
dams and 8 double-sided combs
CSL-RVMSCHOICE10
runVIEW system complete with 15 x 10cm gel tray, 1 set of casting dams and 8 double-sided combs
CSL-RVMSCHOICE15
runVIEW system complete with 15 x 15cm gel tray, 1 set of casting dams and 8 double-sided combs.
CSL-RVMSCHOICETRIO
runVIEW system complete with 15 x 7, 15 x 10 and 15 x 15cm gel trays, 3 sets of casting dams and 8 double-sided combs.